PLASMIDS &
CELL LINES

SnapFast™ Plasmids

OXGENE’s SnapFast™ plasmids are versatile and efficient. These modular plasmids are designed to work like 'molecular building blocks’, using a catalogue of well characterised DNA elements that can be easily and reliably inserted into specific locations within the plasmid, so we can rapidly generate large numbers of custom constructs across a range of expression platforms.

LentiVEX™ Plasmids

OXGENE's LentiVEX™ 3rd generation, self-inactivating (SIN) lentivirus packaging plasmids are optimised for increased translation efficiency, have low homology between expression cassettes for an improved safety profile, and outperform other commercially available equivalents.

Read more about improved transient lentiviral production using LentiVEX™ plasmids

Plasmids for all stages of your therapeutic pipeline

LentiVEX™ plasmids are now available royalty-free at Research, clinical GMP and commercial GMP grade. GMP plasmids are manufactured using a regulatory-friendly, antibiotic free process all the way from initial clone selection through to bacterial banking and GMP manufacture, increasing product purity.

Off-the shelf plasmids are available for international delivery within 2 weeks. Custom plasmids are available for international delivery within 12-20 weeks depending on grade.

We also offer process development and lentiviral production services.

WuXi Advanced Therapies carries out all required quality control assays for bacterial strain bank testing and product release. Testing is integrated throughout the plasmid manufacturing process to ensure high product purity and homogeneity, low endotoxin levels and final product stability.

OXGENE’s LentiVEX™ plasmids are pre-validated for use with the WuXi Advanced Therapies GMP production platforms, alongside their clonal suspension HEK293 cell line. This significantly reduces tech transfer and the timeline to GMP manufacture.

Find out more about our GMP manufacturing platforms

Cell Line Development Services

OXGENE's team of cell line engineering experts design a strategy and use our optimised workflow and high throughput screening platform to maximise project success across multiple applications, including: 

• stable transgene integration and expression

• increased viral vector or biologic expression

• controlled transgene expression or reduced cell toxicity

• disease modelling

Clonal Suspension HEK293 Cell lines

Our clonal suspension HEK293 cell lines are validated for high-titre production of recombinant AAV (rAAV), lentiviral and adenoviral vectors. All cell lines, including engineered and media adapted derivatives, offer full traceability and grow in defined, animal component free media. Cell lines do not contain the SV40 large T antigen, which ensures regulatory compliance of derived products for in vivo applications.

Clonal Suspension HEK293TetR Cell Line

Our GMP compliant suspension HEK293TetR cell line is ideal for amplification of cytotoxic adenoviral vectors. We have demonstrated the functionality of this cell line through our partnership with Vaccitech, who are now using this cell line in clinical trials, and through partner CMOs. In all cases, this animal component and antibiotic free cell line outperformed commercial alternatives. It also demonstrated improved scalability and full stability in the absence of antibiotics and allows optimisation of adenoviral production even for transgenes that reduce cell viability.

We’re also now developing GMP-compliant, suspension mode, stable lentiviral packaging and producer HEK293 cell lines to offer new, industry-leading levels of scalability.
 

Find out more about LentiVEX™ stable cell lines